Single cell type - MLKL - The Human Protein Atlas

MLKL

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GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	MLKL
Gene descriptionⁱ Full gene name according to HGNC.	Mixed lineage kinase domain like pseudokinase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s).	Enzymes Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Adipocytes & Endothelial cells - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Endothelial cells, Adipocytes, Macrophages, Plasma cells)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.59
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Group enriched (Endothelial cell, Central nervous system macrophage, Leukocyte)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Low immune cell specificity
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level

ASSAYSⁱ

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types RNA single cell type specificity: Cell type enhanced (Endothelial cells, Adipocytes, Macrophages, Plasma cells)

TISSUE CELL TYPEⁱ The tissue cell type section provides a cell type specificity prediction for all protein coding genes. More information on the data available can be found here and a description of the analysis here.
	Core cell typesⁱ The core cell type table displays data for a panel of eight cell types that are found in many tissues. Colored dots indicate that the selected gene has core cell type specificity in the indicated tissue. Grey dots indicate that cell type is present within that tissue, but the selected gene is not predicted to be enriched there. Category Alphabetical Enrichment Tissue specific cell typesⁱ This plot shows the enrichment score of the selected gene in all non-core cell types found within the profiled tissues, allowing comparisons between different cell types within different tissues. An enlarged symbol indicates classification of the gene as cell type enriched in the corresponding cell type.

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Group enriched (Endothelial cell, Central nervous system macrophage, Leukocyte)

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas (HPA) data, as well as data generated by (Monaco et al) and (Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Low immune cell specificity
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

MLKL is part of cluster 41 Adipocytes & Endothelial cells - Mixed function with confidenceⁱ 1
325 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
FGD5	FYVE, RhoGEF and PH domain containing 5	0.7636	41
PECAM1	Platelet and endothelial cell adhesion molecule 1	0.7235	41
OAS2	2'-5'-oligoadenylate synthetase 2	0.6234	58
PLEKHO2	Pleckstrin homology domain containing O2	0.6200	58
FAM110D	Family with sequence similarity 110 member D	0.6073	41
PLVAP	Plasmalemma vesicle associated protein	0.6038	41
BCL6B	BCL6B transcription repressor	0.5969	41
ACKR1	Atypical chemokine receptor 1 (Duffy blood group)	0.5956	41
TMEM273	Transmembrane protein 273	0.5952	58
SOX18	SRY-box transcription factor 18	0.5902	41
ADM5	Adrenomedullin 5 (putative)	0.5860	41
CD93	CD93 molecule	0.5808	41
CLEC14A	C-type lectin domain containing 14A	0.5781	41
SOX7	SRY-box transcription factor 7	0.5672	41
ABCG1	ATP binding cassette subfamily G member 1	0.5645	58

IMMUNE CELLS

MLKL is part of cluster 38 Monocytes & Neutrophils - Degranulation with confidenceⁱ 0.98
501 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
C19orf38	Chromosome 19 open reading frame 38	0.9762	38
FKBP1C	FKBP prolyl isomerase family member 1C	0.9762	38
CLK3	CDC like kinase 3	0.9762	38
TOM1	Target of myb1 membrane trafficking protein	0.9762	38
THOC5	THO complex subunit 5	0.9762	38
USP4	Ubiquitin specific peptidase 4	0.9762	38
NINJ2	Ninjurin 2	0.9762	38
SHKBP1	SH3KBP1 binding protein 1	0.9762	38
TMBIM4	Transmembrane BAX inhibitor motif containing 4	0.9762	38
ACTA1	Actin alpha 1, skeletal muscle	0.9762	38
CHMP2A	Charged multivesicular body protein 2A	0.9524	21
SQOR	Sulfide quinone oxidoreductase	0.9524	38
YIPF3	Yip1 domain family member 3	0.9524	21
IFNGR1	Interferon gamma receptor 1	0.9524	38
FGL2	Fibrinogen like 2	0.9524	7

Adipocytes	29.8 nTPM
Adipose tissue	31.0 nTPM
Breast	28.5 nTPM

Alveolar cells type 1	5.0 nTPM
Lung	5.0 nTPM

Alveolar cells type 2	5.2 nTPM
Lung	5.2 nTPM

Astrocytes	0.9 nTPM
Brain	0.9 nTPM

B-cells	7.9 nTPM
Lung	17.4 nTPM
Salivary gland	13.1 nTPM
Esophagus	11.8 nTPM
Thymus	11.0 nTPM
Adipose tissue	9.1 nTPM
Lymph node	7.7 nTPM
Bone marrow	7.2 nTPM
Spleen	6.6 nTPM
Kidney	5.2 nTPM
Stomach	5.1 nTPM
PBMC	4.6 nTPM
Skin	3.4 nTPM
Liver	0.0 nTPM

Ciliated cells	1.2 nTPM
Lung	2.0 nTPM
Bronchus	1.1 nTPM
Fallopian tube	1.0 nTPM
Endometrium	0.9 nTPM

Club cells	3.7 nTPM
Lung	4.9 nTPM
Prostate	4.1 nTPM
Bronchus	2.2 nTPM

Endothelial cells	31.7 nTPM
Fallopian tube	68.7 nTPM
Vascular	59.6 nTPM
Thymus	52.5 nTPM
Salivary gland	41.8 nTPM
Skeletal muscle	41.7 nTPM
Esophagus	40.5 nTPM
Skin	37.8 nTPM
Lung	30.0 nTPM
Endometrium	29.8 nTPM
Ovary	27.7 nTPM
Tongue	24.4 nTPM
Heart muscle	21.5 nTPM
Liver	20.2 nTPM
Prostate	19.8 nTPM
Placenta	15.9 nTPM
Pancreas	15.5 nTPM
Testis	13.4 nTPM
Breast	10.5 nTPM

Enteroendocrine cells	5.2 nTPM
Colon	8.9 nTPM
Small intestine	3.4 nTPM
Rectum	3.4 nTPM

Fibroblasts	5.4 nTPM
Stomach	15.9 nTPM
Liver	11.9 nTPM
Lung	7.0 nTPM
Pancreas	6.8 nTPM
Thymus	6.7 nTPM
Esophagus	6.6 nTPM
Salivary gland	5.7 nTPM
Vascular	5.6 nTPM
Skin	4.2 nTPM
Skeletal muscle	4.2 nTPM
Breast	4.0 nTPM
Adipose tissue	3.0 nTPM
Fallopian tube	2.9 nTPM
Prostate	2.6 nTPM
Heart muscle	0.2 nTPM
Placenta	0.0 nTPM

Granulocytes	15.9 nTPM
Tongue	19.4 nTPM
Lung	16.6 nTPM
Fallopian tube	11.7 nTPM

Intestinal goblet cells	10.6 nTPM
Colon	14.3 nTPM
Small intestine	10.9 nTPM
Rectum	6.7 nTPM

Lymphatic endothelial cells	15.8 nTPM
Ovary	16.4 nTPM
Fallopian tube	15.3 nTPM

Macrophages	25.9 nTPM
Lung	59.8 nTPM
Fallopian tube	55.6 nTPM
Prostate	36.7 nTPM
Thymus	35.6 nTPM
Skeletal muscle	34.0 nTPM
Vascular	29.0 nTPM
Bone marrow	26.4 nTPM
Salivary gland	23.6 nTPM
Pancreas	22.6 nTPM
Spleen	22.1 nTPM
Testis	21.7 nTPM
Endometrium	20.3 nTPM
Kidney	18.6 nTPM
Breast	18.6 nTPM
Adipose tissue	17.7 nTPM
Stomach	15.4 nTPM
Skin	14.5 nTPM
Tongue	11.5 nTPM
Esophagus	7.7 nTPM

Paneth cells	15.1 nTPM
Small intestine	20.7 nTPM
Colon	14.5 nTPM
Rectum	10.0 nTPM

Basal prostatic cells	4.4 nTPM
Prostate	4.4 nTPM

Basal squamous epithelial cells	8.0 nTPM
Esophagus	8.0 nTPM

Cholangiocytes	1.9 nTPM
Liver	1.9 nTPM

Collecting duct cells	1.9 nTPM
Kidney	1.9 nTPM

Plasma cells	24.0 nTPM
Thymus	38.8 nTPM
Salivary gland	34.0 nTPM
Liver	28.5 nTPM
Stomach	26.8 nTPM
Spleen	20.7 nTPM
Bone marrow	18.1 nTPM
Fallopian tube	14.8 nTPM
Esophagus	10.4 nTPM

Schwann cells	2.0 nTPM
Vascular	5.2 nTPM
Adipose tissue	0.8 nTPM
Esophagus	0.0 nTPM

Serous glandular cells	15.5 nTPM
Salivary gland	17.7 nTPM
Tongue	13.3 nTPM

Smooth muscle cells	4.3 nTPM
Liver	17.4 nTPM
Skeletal muscle	12.8 nTPM
Salivary gland	6.0 nTPM
Vascular	4.6 nTPM
Tongue	3.9 nTPM
Esophagus	3.6 nTPM
Thymus	3.2 nTPM
Adipose tissue	3.1 nTPM
Skin	2.6 nTPM
Endometrium	2.3 nTPM
Breast	2.2 nTPM
Fallopian tube	1.8 nTPM
Placenta	1.3 nTPM
Lung	1.2 nTPM
Prostate	1.2 nTPM
Ovary	1.2 nTPM

T-cells	14.7 nTPM
Prostate	25.0 nTPM
Skeletal muscle	23.1 nTPM
Esophagus	21.9 nTPM
Vascular	20.9 nTPM
Breast	19.2 nTPM
Skin	17.9 nTPM
Endometrium	17.4 nTPM
Lung	15.9 nTPM
Adipose tissue	14.9 nTPM
Liver	14.7 nTPM
Tongue	14.2 nTPM
Fallopian tube	14.2 nTPM
Bone marrow	14.0 nTPM
PBMC	11.8 nTPM
Thymus	11.4 nTPM
Salivary gland	10.7 nTPM
Spleen	10.4 nTPM
Lymph node	9.3 nTPM
Colon	8.8 nTPM
Kidney	7.1 nTPM
Stomach	5.5 nTPM

Undifferentiated cells	7.9 nTPM
Colon	9.7 nTPM
Rectum	7.9 nTPM
Small intestine	6.3 nTPM

Amygdala excitatory	0.4 nTPM
Thalamus	0.9 nTPM
Cerebral cortex	0.4 nTPM
Hippocampus	0.4 nTPM
Amygdala	0.3 nTPM
Hypothalamus	0.1 nTPM
Basal ganglia	0.0 nTPM

Astrocyte	0.6 nTPM
Spinal cord	1.5 nTPM
Cerebellum	0.9 nTPM
Basal ganglia	0.6 nTPM
Amygdala	0.5 nTPM
Hippocampus	0.5 nTPM
Medulla oblongata	0.5 nTPM
Midbrain	0.5 nTPM
Cerebral cortex	0.4 nTPM
Hypothalamus	0.4 nTPM
Pons	0.4 nTPM
Thalamus	0.4 nTPM

Bergmann glia	0.7 nTPM
Pons	1.4 nTPM
Cerebellum	0.7 nTPM
Medulla oblongata	0.0 nTPM

Central nervous system macrophage	32.7 nTPM
Amygdala	42.4 nTPM
Hypothalamus	42.3 nTPM
Midbrain	34.5 nTPM
Hippocampus	33.1 nTPM
Cerebellum	32.7 nTPM
Pons	31.7 nTPM
Cerebral cortex	29.5 nTPM
Basal ganglia	29.3 nTPM
Thalamus	29.0 nTPM
Medulla oblongata	28.3 nTPM
Spinal cord	27.1 nTPM

Cerebellar inhibitory	0.3 nTPM
Medulla oblongata	0.7 nTPM
Cerebellum	0.6 nTPM
Pons	0.3 nTPM
Midbrain	0.0 nTPM
Spinal cord	0.0 nTPM

Cge interneuron	0.4 nTPM
Midbrain	1.0 nTPM
Amygdala	0.3 nTPM
Basal ganglia	0.3 nTPM
Cerebral cortex	0.3 nTPM
Hippocampus	0.3 nTPM
Thalamus	0.3 nTPM
Hypothalamus	0.1 nTPM

Choroid plexus epithelial cell	0.5 nTPM
Basal ganglia	1.1 nTPM
Medulla oblongata	1.0 nTPM
Hippocampus	0.0 nTPM
Thalamus	0.0 nTPM

Committed oligodendrocyte precursor	9.8 nTPM
Spinal cord	25.8 nTPM
Hypothalamus	15.0 nTPM
Thalamus	12.6 nTPM
Hippocampus	11.9 nTPM
Amygdala	10.1 nTPM
Cerebral cortex	10.1 nTPM
Basal ganglia	7.1 nTPM
Pons	6.2 nTPM
Medulla oblongata	3.9 nTPM
Cerebellum	3.2 nTPM
Midbrain	1.7 nTPM

Deep-layer corticothalamic and 6b	0.6 nTPM
Midbrain	1.1 nTPM
Hypothalamus	0.7 nTPM
Basal ganglia	0.5 nTPM
Cerebral cortex	0.4 nTPM
Hippocampus	0.4 nTPM
Amygdala	0.3 nTPM

Deep-layer intratelencephalic	0.4 nTPM
Amygdala	0.4 nTPM
Basal ganglia	0.4 nTPM
Cerebral cortex	0.4 nTPM
Hippocampus	0.4 nTPM
Hypothalamus	0.1 nTPM

Deep-layer near-projecting	0.4 nTPM
Hippocampus	0.7 nTPM
Cerebral cortex	0.4 nTPM
Basal ganglia	0.3 nTPM
Amygdala	0.2 nTPM
Hypothalamus	0.2 nTPM

Eccentric medium spiny neuron	0.4 nTPM
Basal ganglia	0.4 nTPM
Hippocampus	0.4 nTPM
Thalamus	0.4 nTPM
Amygdala	0.3 nTPM
Cerebral cortex	0.3 nTPM
Hypothalamus	0.2 nTPM

Endothelial cell	57.5 nTPM
Basal ganglia	208.8 nTPM
Thalamus	65.5 nTPM
Amygdala	51.4 nTPM
Cerebral cortex	47.3 nTPM
Medulla oblongata	45.1 nTPM
Hippocampus	39.4 nTPM
Pons	36.2 nTPM
Spinal cord	36.2 nTPM
Cerebellum	34.8 nTPM
Midbrain	34.0 nTPM
Hypothalamus	33.5 nTPM

Ependymal cell	0.6 nTPM
Hypothalamus	1.8 nTPM
Hippocampus	1.1 nTPM
Medulla oblongata	1.1 nTPM
Basal ganglia	0.8 nTPM
Midbrain	0.6 nTPM
Pons	0.4 nTPM
Amygdala	0.0 nTPM
Spinal cord	0.0 nTPM
Thalamus	0.0 nTPM