The Subcellular resource - A subcellular map of the human proteome
The subcellular resource of the Human Protein Atlas provides high-resolution insights into the expression and spatiotemporal distribution of proteins encoded by 13534 genes (67% of the human protein-coding genes), as well as predictions for an additional 3491 secreted- or membrane proteins, covering a total of 17025 genes (84 % of the human protein-coding genes). For each gene, the subcellular distribution of the protein has been investigated by immunofluorescence (ICC-IF) and confocal microscopy in up to three different standard cell lines, selected from a panel of 41 cell lines used in the subcellular resource. For some genes, the protein has also been stained in up to three ciliated cell lines and/or in human sperm cells. Upon image analysis, the subcellular localization of the protein has been classified into one or more of 49 different organelles and subcellular structures. In addition, the resource includes an annotation of genes that display single-cell variation in protein expression levels and/or subcellular distribution, as well as an extended analysis of cell cycle dependency of such variations.
The subcellular resource offers a database for detailed exploration of individual genes and proteins of interest, as well as for systematic analysis of proteomes in a broader context. More information about the content of the resouce, as well as the generation and analysis of the data, can be found in the Methods summary.
Learn about:
- The subcellular distribution of proteins in human cell lines.
- The subcellular distribution of proteins in human sperm.
- The proteomes of different organelles and subcellular structures.
- Single-cell variability in the expression levels and/or localizations of proteins.