News ArticlesValidation of antibodies using GFP-tagged targetVersion 14 of The Human Protein Atlas includes a new type of validation of antibodies that are used for determining the subcellular localization of a protein. A set of antibodies have been analyzed in transgenic cell lines expressing GFP-tagged target protein at near-endogenous levels to confirm that the antibodies are capable of binding the target protein. The approved antibodies are then used to determine the subcellular localization of endogenous protein in a selection of cell lines. A high validation score is assigned to those genes where the same location(s) are observed for both tagged protein and protein detected using labelled antibody in non-transfected cells...Read more New investment in Life Science researchGreat news for Life Science researchers in Sweden and the new Wallenberg Center for Protein Research (WCPR). AstraZeneca and the Knut and Alice Wallenberg Foundation support a new center for protein research, the Wallenberg Center for Protein Research (WCPR). Three of Swedens top Universities, KTH - Royal Institute of Technology, Uppsala University, and Chalmers Technical Univeristy, will work together to improve human health by developing new biological molecules, antibodies and peptides...Read more Solid-phase cloning for high-throughput assemblySolid-phase cloning for high-throughput assembly of single and multiple DNA parts Simple and automated workflows for DNA assembly are necessary in large-scale projects such as the Human Protein Atlas. For this reason, we have developed two automated methods for swift and convenient high-throughput assembly of DNA into expression vector constructs. By using paramagnetic beads as a support for the DNA, we can perform construct assembly on a liquid handler robot and furthermore eliminate the need for prior purification of PCR products by spin or gel purification...Read more A prognosis based classification of uterin cancerA prognosis based classification of undifferentiated uterine sarcomas: Identification of mitotic index, hormone receptors and YWHAE-FAM22 translocation status as predictors of survival Undifferentiated uterine sarcomas (UUS) are rare tumors with a heterologous biology and a poor prognosis. In a recent study by scientists from the Human Protein Atlas project and colleagues, the relevance of clinicopathology, mitotic index, translocation status (YWHAE-FAM22), and a number of biomarker candidates were examined for correlation with the prognosis of these tumors. The protein biomarkers P53, P16, Ki-67, Cyclin-D1, ER, PR, and ANLN were evaluated by immunohistochemistry...Read more Human Protein Atlas talks and posters in December
Human Quantitative Dynamics Washington DC, USA Presenter: Mathias Uhlén Talk: The Human Protein Atlas Human Quantitative Dynamics
The 7th Annual Meeting of Proteomic Society, India - 2015 Vellore, India Presenter: Prof. Peter Nilsson, KTH Royal Institute of Technology, Sweden Talk: Human Protein Atlas enabled neuroproteomic profiling of body fluids The 7th Annual Meeting of Proteomic Society
Salamanca, Spain Dr. Jochen M...Read more |