Expression of VEGFB in glioma - The Human Protein Atlas

GLIOBLASTOMA MULTIFORME (TCGA) - Interactive survival scatter plotⁱ

The Survival Scatter plot shows the clinical status (i.e. dead or alive) for all individuals in the patient cohort, based on the same data that underlies the corresponding Kaplan-Meier plots. Patients that are alive at last time for follow-up are shown in blue and patients who have died during the study are shown in red.

The x-axis shows the expression levels (FPKM) of the investigated gene in the tumor tissue at the time of diagnosis. The y-axis shows the follow-up time after diagnosis (years). Both axes are complimented with kernel density curves demonstrating the data density over the axes. The top density plot shows the expression levels (FPKM) distribution among dead (red) and alive patients (blue). The right density plot shows the data density of the survived years of dead patients with high and low expression levels respectively, stratified using the cutoff indicated by the vertical dashed line through the Survival Scatter plot. This cutoff is automatically defined based on the FPKM cutoff that minimizes the p-score. The cutoff can be changed by dragging the vertical line or by entering a cutoff value in the square labeled "Current cut-off".

Under the Survival Scatter plot the p-score landscape (black curve; left axis) is shown together with dead median separation (red curve; right axis). Dead median separation is the difference in median mRNA expression between patients who have died with high and low expression, respectively. It is calculated as follows: median FPKM expression of dead patients with high expression - median FPKM expression of dead patients with low expression. This is intended to aid the user in visually exploring custom cutoffs and the associated p-scores and dead median separation.

Individual patient data is displayed and can be filtered by clicking on one or more of the category buttons on the top of the page. Categories describing expression level and patient information include: high, low, alive, dead, female, male and tumor stages. The scale of the x-axis can be toggled between linear and log-scale by clicking on the "x log" button. Mouse-over function shows TCGA ID, patient information and mRNA expression (FPKM) for each patient.

& Survival analysisⁱ

VEGFB is not prognostic in Glioblastoma Multiforme (TCGA)

Stage:

Survival analysis

Current cut offⁱ :

Best expression cut offⁱ : 71.35

Median expressionⁱ : N/A

Median follow up timeⁱ : N/A

P scoreⁱ N/A

3-year survival highⁱ N/A

3-year survival lowⁱ N/A

TCGA RNA samplesⁱ

Average TPM

57.5

Number of samples

141

Samples

Sample	Description	TPM
TCGA-32-2616-01A	0 years, female, white, stage:'--, dead, 224 days	140.2
TCGA-06-0745-01A	0 years, male, white, stage:'--, dead, 239 days	109.9
TCGA-15-0742-01A	0 years, male, white, stage:'--, dead, 419 days	105.0
TCGA-26-5132-01A	0 years, male, white, stage:'--, alive, 286 days	100.4
TCGA-41-2572-01A	0 years, male, white, stage:'--, dead, 406 days	99.8
TCGA-12-3653-01A	0 years, female, white, stage:'--, dead, 442 days	92.5
TCGA-06-0749-01A	0 years, male, black or african american, stage:'--, dead, 82 days	85.8
TCGA-12-0618-01A	0 years, male, white, stage:'--, dead, 395 days	84.1
TCGA-06-0750-01A	0 years, male, white, stage:'--, dead, 28 days	83.8
TCGA-06-0210-01A	0 years, female, white, stage:'--, dead, 225 days	83.3
TCGA-76-4925-01A	0 years, male, white, stage:'--, dead, 146 days	82.9
TCGA-08-0386-01A	0 years, male, white, stage:'--, dead, 548 days	82.4
TCGA-27-2521-01A	0 years, male, white, stage:'--, dead, 510 days	79.7
TCGA-06-0187-01A	0 years, male, white, stage:'--, dead, 828 days	79.5
TCGA-12-5295-01A	0 years, female, white, stage:'--, dead, 454 days	77.4
TCGA-06-2557-01A	0 years, male, black or african american, stage:'--, dead, 33 days	77.2
TCGA-19-2625-01A	0 years, female, white, stage:'--, dead, 124 days	77.0
TCGA-14-0787-01A	0 years, male, asian, stage:'--, dead, 68 days	76.8
TCGA-28-5220-01A	0 years, male, white, stage:'--, dead, 388 days	76.7
TCGA-19-2629-01A	0 years, male, white, stage:'--, dead, 737 days	75.9
TCGA-12-3650-01A	0 years, male, white, stage:'--, dead, 333 days	75.8
TCGA-26-5135-01A	0 years, female, white, stage:'--, dead, 270 days	75.7
TCGA-12-5299-01A	0 years, female, white, stage:'--, dead, 98 days	75.4
TCGA-28-5204-01A	0 years, male, white, stage:'--, dead, 454 days	75.0
TCGA-28-2509-01A	0 years, female, white, stage:'--, alive, 145 days	74.4
TCGA-06-0646-01A	0 years, male, white, stage:'--, dead, 175 days	73.7
TCGA-26-5133-01A	0 years, male, white, stage:'--, alive, 452 days	72.8
TCGA-06-0644-01A	0 years, male, black or african american, stage:'--, dead, 384 days	72.5
TCGA-06-5418-01A	0 years, female, white, stage:'--, dead, 83 days	71.5
TCGA-06-0184-01A	0 years, male, white, stage:'--, dead, 2126 days	71.2
TCGA-41-4097-01A	0 years, female, white, stage:'--, dead, 6 days	70.3
TCGA-19-2619-01A	0 years, female, black or african american, stage:'--, alive, 294 days	70.3
TCGA-76-4931-01A	0 years, female, white, stage:'--, dead, 279 days	70.2
TCGA-06-0168-01A	0 years, female, white, stage:'--, dead, 598 days	70.2
TCGA-27-2523-01A	0 years, male, white, stage:'--, dead, 489 days	70.0
TCGA-12-3652-01A	0 years, male, white, stage:'--, dead, 1062 days	69.1
TCGA-06-0686-01A	0 years, male, white, stage:'--, dead, 432 days	69.0
TCGA-06-5408-01A	0 years, female, white, stage:'--, dead, 357 days	68.8
TCGA-06-2567-01A	0 years, male, white, stage:'--, dead, 133 days	67.9
TCGA-06-0157-01A	0 years, female, white, stage:'--, dead, 97 days	67.7
TCGA-32-2634-01A	0 years, male, white, stage:'--, alive, 693 days	67.0
TCGA-27-1834-01A	0 years, male, white, stage:'--, dead, 1233 days	66.8
TCGA-06-0158-01A	0 years, male, white, stage:'--, dead, 329 days	66.7
TCGA-06-5858-01A	0 years, female, white, stage:'--, alive, 187 days	66.5
TCGA-06-1804-01A	0 years, female, white, stage:'--, dead, 414 days	66.5
TCGA-27-1835-01A	0 years, female, white, stage:'--, dead, 648 days	66.2
TCGA-15-1444-01A	0 years, male, white, stage:'--, dead, 1537 days	66.1
TCGA-06-0882-01A	0 years, male, white, stage:'--, dead, 632 days	64.7
TCGA-02-2486-01A	0 years, male, white, stage:'--, dead, 618 days	64.3
TCGA-19-1390-01A	0 years, female, white, stage:'--, dead, 772 days	63.9
TCGA-32-1970-01A	0 years, male, white, stage:'--, dead, 468 days	63.8
TCGA-41-2571-01A	0 years, male, white, stage:'--, dead, 26 days	63.6
TCGA-06-0130-01A	0 years, male, white, stage:'--, dead, 394 days	63.5
TCGA-06-0156-01A	0 years, male, white, stage:'--, dead, 178 days	63.1
TCGA-76-4932-01A	0 years, female, white, stage:'--, dead, 1458 days	62.3
TCGA-06-2563-01A	0 years, female, white, stage:'--, alive, 932 days	62.1
TCGA-27-2526-01A	0 years, female, white, stage:'--, dead, 87 days	61.5
TCGA-06-2564-01A	0 years, male, white, stage:'--, alive, 181 days	61.3
TCGA-12-0821-01A	0 years, male, white, stage:'--, dead, 323 days	60.8
TCGA-14-1825-01A	0 years, male, white, stage:'--, dead, 232 days	60.4
TCGA-06-0174-01A	0 years, male, white, stage:'--, dead, 98 days	60.2
TCGA-41-3915-01A	0 years, male, white, stage:'--, dead, 360 days	60.2
TCGA-06-0211-01A	0 years, male, white, stage:'--, dead, 360 days	60.1
TCGA-19-2624-01A	0 years, male, white, stage:'--, dead, 5 days	60.0
TCGA-32-2615-01A	0 years, male, white, stage:'--, dead, 485 days	59.7
TCGA-06-0219-01A	0 years, male, white, stage:'--, dead, 22 days	59.2
TCGA-32-2638-01A	0 years, male, white, stage:'--, dead, 766 days	57.8
TCGA-02-0047-01A	0 years, male, white, stage:'--, dead, 448 days	57.4
TCGA-02-0055-01A	0 years, female, white, stage:'--, dead, 76 days	57.1
TCGA-06-0743-01A	0 years, male, white, stage:'--, dead, 803 days	57.1
TCGA-28-2513-01A	0 years, female, white, stage:'--, alive, 222 days	56.8
TCGA-28-5209-01A	0 years, female, white, stage:'--, alive, 442 days	55.5
TCGA-06-5414-01A	0 years, male, white, stage:'--, alive, 273 days	55.4
TCGA-06-0744-01A	0 years, male, white, stage:'--, dead, 1426 days	55.3
TCGA-06-0129-01A	0 years, male, asian, stage:'--, dead, 1024 days	54.7
TCGA-28-1753-01A	0 years, male, white, stage:'--, alive, 37 days	54.1
TCGA-06-0878-01A	0 years, male, white, stage:'--, alive, 218 days	54.1
TCGA-06-0747-01A	0 years, male, white, stage:'--, dead, 82 days	53.6
TCGA-02-2483-01A	0 years, male, asian, stage:'--, alive, 466 days	53.5
TCGA-76-4929-01A	0 years, female, white, stage:'--, dead, 111 days	53.1
TCGA-06-0125-01A	0 years, female, white, stage:'--, dead, 1448 days	52.6
TCGA-14-1034-01A	0 years, female, stage:'--, dead, 485 days	52.4
TCGA-28-2514-01A	0 years, male, asian, stage:'--, alive, 160 days	52.3
TCGA-06-5412-01A	0 years, female, white, stage:'--, dead, 138 days	51.3
TCGA-06-0132-01A	0 years, male, white, stage:'--, dead, 771 days	50.9
TCGA-06-0645-01A	0 years, female, white, stage:'--, dead, 175 days	50.6
TCGA-76-4927-01A	0 years, male, white, stage:'--, dead, 535 days	49.9
TCGA-06-2559-01A	0 years, male, white, stage:'--, dead, 150 days	49.7
TCGA-06-5410-01A	0 years, female, white, stage:'--, dead, 108 days	49.6
TCGA-14-0789-01A	0 years, male, white, stage:'--, dead, 342 days	48.9
TCGA-27-2524-01A	0 years, male, white, stage:'--, dead, 231 days	48.7
TCGA-06-2561-01A	0 years, female, white, stage:'--, dead, 537 days	48.5
TCGA-32-2632-01A	0 years, male, white, stage:'--, dead, 269 days	48.4
TCGA-27-2528-01A	0 years, male, white, stage:'--, dead, 480 days	48.2
TCGA-06-2570-01A	0 years, female, white, stage:'--, alive, 958 days	48.2
TCGA-27-2519-01A	0 years, male, white, stage:'--, dead, 550 days	48.1
TCGA-26-1442-01A	0 years, male, white, stage:'--, alive, 953 days	47.6
TCGA-32-4213-01A	0 years, female, white, stage:'--, alive, 604 days	47.4
TCGA-06-0141-01A	0 years, male, white, stage:'--, dead, 313 days	47.3
TCGA-06-2562-01A	0 years, male, white, stage:'--, dead, 382 days	47.3
TCGA-27-1832-01A	0 years, female, white, stage:'--, dead, 300 days	47.3
TCGA-27-1837-01A	0 years, male, white, stage:'--, dead, 427 days	47.2
TCGA-12-0619-01A	0 years, male, white, stage:'--, dead, 1062 days	47.1
TCGA-06-0139-01A	0 years, male, white, stage:'--, dead, 362 days	46.3
TCGA-28-5216-01A	0 years, male, white, stage:'--, alive, 415 days	46.0
TCGA-06-5411-01A	0 years, male, white, stage:'--, dead, 254 days	45.5
TCGA-32-1982-01A	0 years, female, white, stage:'--, dead, 142 days	45.3
TCGA-19-2620-01A	0 years, male, white, stage:'--, dead, 148 days	44.7
TCGA-26-5139-01A	0 years, female, white, stage:'--, alive, 48 days	44.6
TCGA-32-1980-01A	0 years, male, white, stage:'--, dead, 36 days	44.5
TCGA-28-5215-01A	0 years, female, white, stage:'--, dead, 335 days	44.3
TCGA-06-5416-01A	0 years, female, white, stage:'--, alive, 204 days	44.0
TCGA-06-5413-01A	0 years, male, white, stage:'--, alive, 268 days	43.6
TCGA-06-0138-01A	0 years, male, white, stage:'--, dead, 737 days	43.0
TCGA-06-2569-01A	0 years, female, black or african american, stage:'--, alive, 13 days	42.8
TCGA-06-2565-01A	0 years, male, asian, stage:'--, dead, 506 days	41.4
TCGA-06-2558-01A	0 years, female, white, stage:'--, dead, 380 days	40.8
TCGA-06-5856-01A	0 years, male, white, stage:'--, dead, 114 days	40.0
TCGA-28-5208-01A	0 years, male, white, stage:'--, dead, 544 days	38.9
TCGA-41-5651-01A	0 years, female, black or african american, stage:'--, dead, 460 days	37.6
TCGA-02-2485-01A	0 years, male, black or african american, stage:'--, alive, 470 days	36.4
TCGA-12-0616-01A	0 years, female, white, stage:'--, dead, 448 days	36.1
TCGA-27-1830-01A	0 years, male, white, stage:'--, dead, 154 days	35.5
TCGA-06-5859-01A	0 years, male, white, stage:'--, alive, 139 days	35.1
TCGA-32-5222-01A	0 years, male, white, stage:'--, dead, 585 days	35.1
TCGA-14-0817-01A	0 years, female, white, stage:'--, dead, 164 days	34.9
TCGA-14-0871-01A	0 years, female, white, stage:'--, dead, 880 days	34.8
TCGA-28-5218-01A	0 years, male, white, stage:'--, dead, 157 days	32.3
TCGA-14-1823-01A	0 years, female, white, stage:'--, dead, 543 days	31.5
TCGA-06-0190-01A	0 years, male, white, stage:'--, dead, 317 days	31.0
TCGA-16-0846-01A	0 years, male, white, stage:'--, dead, 119 days	30.8
TCGA-28-5207-01A	0 years, male, white, stage:'--, dead, 343 days	29.0
TCGA-19-4065-01A	0 years, male, white, stage:'--, alive, 214 days	29.0
TCGA-06-5417-01A	0 years, female, white, stage:'--, alive, 155 days	28.9
TCGA-14-2554-01A	0 years, female, white, stage:'--, dead, 532 days	28.8
TCGA-06-0238-01A	0 years, male, white, stage:'--, dead, 405 days	27.9
TCGA-06-0178-01A	0 years, male, white, stage:'--, dead, 2681 days	25.6
TCGA-27-1831-01A	0 years, male, white, stage:'--, dead, 505 days	25.0
TCGA-14-1829-01A	0 years, male, black or african american, stage:'--, alive, 218 days	24.7
TCGA-26-5134-01A	0 years, male, white, stage:'--, alive, 167 days	22.7
TCGA-19-5960-01A	0 years, male, white, stage:'--, dead, 455 days	21.4
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GLIOBLASTOMA MULTIFORME (VALIDATION) - Interactive survival scatter plotⁱ

& Survival analysisⁱ

VEGFB is not prognostic in Glioblastoma Multiforme (validation)

Stage:

Survival analysis

Current cut offⁱ :

Best expression cut offⁱ : 16.84

Median expressionⁱ : N/A

Median follow up timeⁱ : N/A

P scoreⁱ N/A

3-year survival highⁱ N/A

3-year survival lowⁱ N/A

TCGA RNA samplesⁱ

Average TPM

16.2

Number of samples

Samples

Sample	Description	TPM
AK068	N, NA, stage:NA, alive, 360 days	46.0
AK088	N, NA, stage:NA, dead, 360 days	32.8
AK153	N, NA, stage:NA, dead, 240 days	31.6
AK072	N, NA, stage:NA, dead, 150 days	27.5
AK043	N, NA, stage:NA, alive, 660 days	24.7
AK213	N, NA, stage:NA, alive, 2010 days	23.2
AK085	N, NA, stage:NA, alive, 660 days	20.6
AK167	N, NA, stage:NA, dead, 180 days	20.2
AK055	N, NA, stage:NA, dead, 240 days	19.9
AK091	N, NA, stage:NA, dead, 210 days	19.2
AK134	N, NA, stage:NA, dead, 360 days	19.2
AK030	N, NA, stage:NA, dead, 120 days	19.2
AK205	N, NA, stage:NA, dead, 120 days	19.1
AK236	N, NA, stage:NA, dead, 360 days	18.6
AK178	N, NA, stage:NA, dead, 240 days	18.0
AK218	N, NA, stage:NA, dead, 180 days	17.9
AK081	N, NA, stage:NA, dead, 180 days	17.6
AK216	N, NA, stage:NA, dead, 360 days	17.4
AK002	N, NA, stage:NA, dead, 570 days	17.3
AK035	N, NA, stage:NA, dead, 210 days	17.2
AK051	N, NA, stage:NA, dead, 360 days	17.0
AK227	N, NA, stage:NA, alive, 300 days	16.7
AK006	N, NA, stage:NA, dead, 360 days	16.6
AK183	N, NA, stage:NA, alive, 360 days	16.5
AK005	N, NA, stage:NA, dead, 240 days	16.4
AK195	N, NA, stage:NA, dead, 390 days	16.3
AK041	N, NA, stage:NA, alive, 810 days	16.2
AK089	N, NA, stage:NA, dead, 330 days	16.0
AK079	N, NA, stage:NA, dead, 210 days	15.6
AK015	N, NA, stage:NA, alive, 720 days	15.4
AK099	N, NA, stage:NA, dead, 420 days	15.1
AK133	N, NA, stage:NA, dead, 360 days	15.0
AK076	N, NA, stage:NA, alive, 660 days	14.9
AK226	N, NA, stage:NA, dead, 360 days	14.8
AK124	N, NA, stage:NA, alive, 1920 days	14.5
AK231	N, NA, stage:NA, dead, 240 days	14.5
AK098	N, NA, stage:NA, dead, 660 days	14.3
AK074	N, NA, stage:NA, alive, 300 days	14.0
AK158	N, NA, stage:NA, dead, 360 days	13.9
AK185	N, NA, stage:NA, alive, 330 days	13.9
AK117	N, NA, stage:NA, dead, 210 days	13.7
AK053	N, NA, stage:NA, dead, 300 days	13.5
AK123	N, NA, stage:NA, dead, 180 days	13.3
AK188	N, NA, stage:NA, alive, 420 days	13.0
AK071	N, NA, stage:NA, dead, 540 days	12.9
AK100	N, NA, stage:NA, alive, 960 days	11.9
AK003	N, NA, stage:NA, dead, 600 days	11.3
AK149	N, NA, stage:NA, dead, 420 days	11.3
AK142	N, NA, stage:NA, alive, 90 days	10.5
AK139	N, NA, stage:NA, dead, 270 days	10.2
AK103	N, NA, stage:NA, alive, 1320 days	9.2
AK173	N, NA, stage:NA, dead, 180 days	8.7
AK199	N, NA, stage:NA, alive, 1860 days	8.6
AK066	N, NA, stage:NA, alive, 960 days	8.5
AK049	N, NA, stage:NA, dead, 180 days	8.5
AK156	N, NA, stage:NA, dead, 330 days	8.3
AK102	N, NA, stage:NA, alive, 1740 days	7.3
AK165	N, NA, stage:NA, alive, 360 days	6.5
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GLIOBLASTOMA MULTIFORME - Protein relative expression (CPTAC)

Number of samples

110

Samples

Sample ID	Sample type	nRPX
CPT0206330003	Tumor	N/A
CPT0186100003	Tumor	N/A
CPT0123530003	Tumor	N/A
CPT0201710003	Tumor	N/A
CPT0216920008	Tumor	N/A
CPT0218890004	Tumor	N/A
CPT0224390004	Tumor	N/A
CPT0204390003	Normal	N/A
CPT0093590003	Tumor	N/A
CPT0167750004	Tumor	N/A
CPT0204400003	Normal	N/A
CPT0079790003	Tumor	N/A
CPT0218690004	Tumor	N/A
CPT0204370003	Normal	N/A
CPT0161730003	Tumor	N/A
CPT0168720003	Tumor	N/A
CPT0206880003	Tumor	N/A
CPT0189650004	Tumor	N/A
CPT0204350003	Normal	N/A
CPT0168380003	Tumor	N/A
CPT0205890003	Tumor	N/A
CPT0217880003	Tumor	N/A
CPT0206560003	Tumor	N/A
CPT0087680003	Tumor	N/A
CPT0206000004	Tumor	N/A
CPT0218670003	Tumor	N/A
CPT0204340003	Normal	N/A
CPT0189850004	Tumor	N/A
CPT0224330003	Tumor	N/A
CPT0218330004	Tumor	N/A
CPT0167640003	Tumor	N/A
CPT0002410011	Tumor	N/A
CPT0093450003	Tumor	N/A
CPT0206110003	Tumor	N/A
CPT0217430008	Tumor	N/A
CPT0217000004	Tumor	N/A
CPT0196850003	Tumor	N/A
CPT0189250003	Tumor	N/A
CPT0189570004	Tumor	N/A
CPT0162020003	Tumor	N/A
CPT0167530003	Tumor	N/A
CPT0207030003	Tumor	N/A
CPT0127420003	Tumor	N/A
CPT0205670004	Tumor	N/A
CPT0208980003	Tumor	N/A
CPT0206780003	Tumor	N/A
CPT0182500003	Tumor	N/A
CPT0217710008	Tumor	N/A
CPT0168830003	Tumor	N/A
CPT0182550003	Tumor	N/A
CPT0189460003	Tumor	N/A
CPT0167860004	Tumor	N/A
CPT0125220003	Tumor	N/A
CPT0171580008	Tumor	N/A
CPT0087730003	Tumor	N/A
CPT0104330003	Tumor	N/A
CPT0168590003	Tumor	N/A
CPT0205450004	Tumor	N/A
CPT0219080004	Tumor	N/A
CPT0087570003	Tumor	N/A
CPT0162140003	Tumor	N/A
CPT0175060003	Tumor	N/A
CPT0205780003	Tumor	N/A
CPT0218960004	Tumor	N/A
CPT0064650003	Tumor	N/A
CPT0125570003	Tumor	N/A
CPT0190360004	Tumor	N/A
CPT0206670004	Tumor	N/A
CPT0204380003	Normal	N/A
CPT0092440003	Tumor	N/A
CPT0162100003	Tumor	N/A
CPT0221180003	Tumor	N/A
CPT0225760003	Tumor	N/A
CPT0093510003	Tumor	N/A
CPT0190240004	Tumor	N/A
CPT0228220003	Tumor	N/A
CPT0204410003	Normal	N/A
CPT0224540004	Tumor	N/A
CPT0209440003	Tumor	N/A
CPT0089150003	Tumor	N/A
CPT0204360003	Normal	N/A
CPT0168080003	Tumor	N/A
CPT0078580003	Tumor	N/A
CPT0127480003	Tumor	N/A
CPT0204420003	Normal	N/A
CPT0218830004	Tumor	N/A
CPT0225730003	Tumor	N/A
CPT0093360003	Tumor	N/A
CPT0168270003	Tumor	N/A
CPT0206450003	Tumor	N/A
CPT0207090003	Tumor	N/A
CPT0104220003	Tumor	N/A
CPT0224600003	Tumor	N/A
CPT0087950003	Tumor	N/A
CPT0204330003	Normal	N/A
CPT0199770003	Tumor	N/A
CPT0205570003	Tumor	N/A
CPT0093550003	Tumor	N/A
CPT0167970003	Tumor	N/A
CPT0182580003	Tumor	N/A
CPT0168480003	Tumor	N/A
CPT0206230003	Tumor	N/A
CPT0189750004	Tumor	N/A
CPT0217100003	Tumor	N/A
CPT0064890003	Tumor	N/A
CPT0217190003	Tumor	N/A
CPT0218770003	Tumor	N/A
CPT0125510003	Tumor	N/A
CPT0217060003	Tumor	N/A
CPT0071100003	Tumor	N/A
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GLIOMA - Protein expressionⁱ

Pending cancer tissue analysis