Subcellular - SLC16A1 - The Human Protein Atlas

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SLC16A1

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Field

Cluster

Pathway

SUMMARY

TISSUE

BRAIN

SINGLE CELL

SUBCELL

CANCER

BLOOD

CELL LINE

STRUCT & INT

SLC16A1

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GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	SLC16A1
Gene descriptionⁱ Full gene name according to HGNC.	Solute carrier family 16 member 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s).	Disease related genes Human disease related genes Metabolic proteins Potential drug targets Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Main locationⁱ Main subcellular location(s) and reliability score(s) for the encoded protein(s) in human cells. The main location(s) may be characterized by presence in all tested cell lines and/or ihigher staining intensity compared to the potential additional location(s). If available, links to overrepresentation analyses in Reactome, a free, open-source, curated and peer reviewed biological pathway database, are provided. An analysis is done for the corresponding gene set of the proteome localizing to the main and additional locations of the protein on this page, respectively.	Localized to the Plasma membrane (enhanced), Cell Junctions (enhanced)
Single-cell variationⁱ Single-cell variations in protein expression levels or distribution observed between cells within the same images of human cells stained by immunocytochemistry.	Single-cell variation in protein expression observed.
siRNAⁱ Validation score for the subcellular location(s) of the encoded proteins(s) according to siRNA validation.	The location(s) are Enhanced based on siRNA mediated gene silencing.
Reliability scoreⁱ Overall gene reliability score for the subcellular location(s) of the encoded protein(s). The score indicates the level of reliability of the annotated protein localization based on agreement with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database and presence of antibody validation assays for immunocytochemistry.	Enhanced
Antibodiesⁱ Antibodies used for this assay. Click on an antibody for more information.	HPA003324 , CAB017489 , HPA071055
	SHOW MORE

ASSAYSⁱ

Summaryⁱ

Mainly localized to the plasma membrane and cell junctions.

Main locationⁱ

Plasma membrane (enhanced), Cell Junctions (enhanced)

Human cell lines

siRNA

RNA expression

HUMAN CELL LINESⁱ


	Toggle channelsⁱ Buttons for turning on and off the different channels in the multi-color images. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel. The object toggle shows the computational segmentation of the cells used for further analysis in the HPA project. For samples where cell cycle dependency for the protein is suggested according to a correlation assay the predicted cell cycle position of each cell is displayed when using the object toggle.	Low High

Antibodyⁱ Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.	Cell lineⁱ Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.	Cell line RNA Expression (nTPM)ⁱ RNA expression level in the cell line based on normalised RNA sequencing data. Genes with a value above 1 NX are considered to be expressed.	Locationⁱ Observed subcellular location(s).	Single-cell variationⁱ Observed single-cell variation(s) in the staining pattern. Variation in protein expression levels can be observed as varaiations in the intensity of the immunofluorescent signal, while spatial variations can be observed as variations in subcellular distribution of the protein.	Cell cycle dependent variationⁱ Cell-cycle dependance of observed single-cell varations in RNA and/or protein expression as observed in an additional assay for characterizing single-cell varations.
CAB017489	A-431	108.1	Plasma membrane
CAB017489	A-431	108.1	Microtubules
CAB017489	U-251MG	136.7	Plasma membrane
CAB017489	U2OS	49.7	Plasma membrane
			Microtubules
			Cytokinetic bridge	Yes	Intensity
HPA003324	A-431	108.1	Plasma membrane	Yes
HPA003324	U-251MG	136.7	Plasma membrane
HPA003324	U2OS	49.7	Plasma membrane
HPA071055	HaCaT	107.1	Vesicles
			Plasma membrane
			Cell Junctions
HPA071055	U2OS	49.7	Vesicles
			Plasma membrane
			Cell Junctions

siRNA VALIDATIONⁱ

Antibodyⁱ

HPA003324

siRNA validationⁱ

Enhanced: Significant downregulation > 25% by one siRNA.

Analysis performed inⁱ

Cytoplasmic region of segmented cells in 10x-images

siRNA 2ⁱ

RFIⁱ

40%

p-valueⁱ

< 0.01


	Toggle channelsⁱ Buttons for turning on and off the different channels in the multi-color images. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel. The antibody and intensity channel toggle can be used to further illustrate the intensity downregulation when comparing images from scrambled and targeting siRNA samples in the images. The object toggle shows the segmentation compartment in which the change in intensity after siRNA downregulation was measured.	Low High

Thumbnail 10xⁱ Minatures of all available images acquired with a 10x objective after siRNA downregulation. The checkboxes can be used to select which three images to compare in the window above. All images are also clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image.	Thumbnail 40xⁱ Minatures of all available images acquired with a 40x objective after siRNA downregulation. The checkboxes can be used to select which three images to compare in the window above. All images are also clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image.	Antibodyⁱ Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.	Sampleⁱ siRNA that was used for the analysis.	Cell lineⁱ Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.
		HPA003324	siRNA 2	U2OS
		HPA003324	scrambled	U2OS

RNA EXPRESSION OVERVIEWⁱ

Organ

Origin