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Strong intensity immunostaining of axonal networks is noticed in the caudate putamen, nucleus accumbens (core and shell), olfactory tubercule, ventral pallidum, thalamic paraventricular nucleus and the hypothalamic arcuate nucleus. General weak-moderate intensity immunostaining of axonal networks is noticed in all other regions of the olfactory bulb and forebrain.
In the midbrain and pons, the weak-moderate intensity staining of axons is rather limited to certain nuceli/regions, like the substantia nigra - ventral tegmental area and the periaqueductal gray matter; other regions exhibit only very sparse axonal staining.
In the medulla, the axonal staining is more widespread and general again, with higher densities in the locus coeruleus-parabrachial- and the n. tractus solitarii regions.
The cerebellum does not exhibit any immunostaining in any layers and cell types.
The median eminence and area postrema (circumventricular organs) are strongly immunoreactive just like the ventricle walls and the wall of bigger vessels around the brain.
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Positive cells and structuresi
Manually selected location of the protein positivity, observed by immunofluorescence staining in mouse brain.
Axon in neurons. Choroid plexus, ventricle wall and circumventricular organs of ependymal cells.
Positivity observed in processes in caudate putamen