Expression of OLR1 in glioma - The Human Protein Atlas

GLIOBLASTOMA MULTIFORME (TCGA) - Interactive survival scatter plotⁱ

The Survival Scatter plot shows the clinical status (i.e. dead or alive) for all individuals in the patient cohort, based on the same data that underlies the corresponding Kaplan-Meier plots. Patients that are alive at last time for follow-up are shown in blue and patients who have died during the study are shown in red.

The x-axis shows the expression levels (FPKM) of the investigated gene in the tumor tissue at the time of diagnosis. The y-axis shows the follow-up time after diagnosis (years). Both axes are complimented with kernel density curves demonstrating the data density over the axes. The top density plot shows the expression levels (FPKM) distribution among dead (red) and alive patients (blue). The right density plot shows the data density of the survived years of dead patients with high and low expression levels respectively, stratified using the cutoff indicated by the vertical dashed line through the Survival Scatter plot. This cutoff is automatically defined based on the FPKM cutoff that minimizes the p-score. The cutoff can be changed by dragging the vertical line or by entering a cutoff value in the square labeled "Current cut-off".

Under the Survival Scatter plot the p-score landscape (black curve; left axis) is shown together with dead median separation (red curve; right axis). Dead median separation is the difference in median mRNA expression between patients who have died with high and low expression, respectively. It is calculated as follows: median FPKM expression of dead patients with high expression - median FPKM expression of dead patients with low expression. This is intended to aid the user in visually exploring custom cutoffs and the associated p-scores and dead median separation.

Individual patient data is displayed and can be filtered by clicking on one or more of the category buttons on the top of the page. Categories describing expression level and patient information include: high, low, alive, dead, female, male and tumor stages. The scale of the x-axis can be toggled between linear and log-scale by clicking on the "x log" button. Mouse-over function shows TCGA ID, patient information and mRNA expression (FPKM) for each patient.

& Survival analysisⁱ

OLR1 is not prognostic in Glioblastoma Multiforme (TCGA)

Stage:

Survival analysis

Current cut offⁱ :

Best expression cut offⁱ : 17.01

Median expressionⁱ : N/A

Median follow up timeⁱ : N/A

P scoreⁱ N/A

3-year survival highⁱ N/A

3-year survival lowⁱ N/A

TCGA RNA samplesⁱ

Average TPM

26.2

Number of samples

141

Samples

Sample	Description	TPM
TCGA-06-0129-01A	0 years, male, asian, stage:'--, dead, 1024 days	113.9
TCGA-32-5222-01A	0 years, male, white, stage:'--, dead, 585 days	112.7
TCGA-06-2564-01A	0 years, male, white, stage:'--, alive, 181 days	108.2
TCGA-06-0139-01A	0 years, male, white, stage:'--, dead, 362 days	100.6
TCGA-06-0749-01A	0 years, male, black or african american, stage:'--, dead, 82 days	76.4
TCGA-06-0130-01A	0 years, male, white, stage:'--, dead, 394 days	72.9
TCGA-15-1444-01A	0 years, male, white, stage:'--, dead, 1537 days	72.0
TCGA-06-2561-01A	0 years, female, white, stage:'--, dead, 537 days	71.1
TCGA-02-2486-01A	0 years, male, white, stage:'--, dead, 618 days	67.8
TCGA-06-2557-01A	0 years, male, black or african american, stage:'--, dead, 33 days	65.7
TCGA-27-1834-01A	0 years, male, white, stage:'--, dead, 1233 days	65.1
TCGA-06-0646-01A	0 years, male, white, stage:'--, dead, 175 days	65.0
TCGA-06-5858-01A	0 years, female, white, stage:'--, alive, 187 days	60.7
TCGA-06-0882-01A	0 years, male, white, stage:'--, dead, 632 days	58.9
TCGA-06-0878-01A	0 years, male, white, stage:'--, alive, 218 days	55.0
TCGA-06-0158-01A	0 years, male, white, stage:'--, dead, 329 days	54.9
TCGA-32-2632-01A	0 years, male, white, stage:'--, dead, 269 days	47.4
TCGA-06-5410-01A	0 years, female, white, stage:'--, dead, 108 days	45.9
TCGA-28-5207-01A	0 years, male, white, stage:'--, dead, 343 days	44.5
TCGA-16-0846-01A	0 years, male, white, stage:'--, dead, 119 days	44.1
TCGA-06-0184-01A	0 years, male, white, stage:'--, dead, 2126 days	43.7
TCGA-06-0238-01A	0 years, male, white, stage:'--, dead, 405 days	42.6
TCGA-32-1980-01A	0 years, male, white, stage:'--, dead, 36 days	42.6
TCGA-28-5215-01A	0 years, female, white, stage:'--, dead, 335 days	41.6
TCGA-06-5412-01A	0 years, female, white, stage:'--, dead, 138 days	41.5
TCGA-06-2567-01A	0 years, male, white, stage:'--, dead, 133 days	38.2
TCGA-06-5414-01A	0 years, male, white, stage:'--, alive, 273 days	38.1
TCGA-14-1034-01A	0 years, female, stage:'--, dead, 485 days	37.8
TCGA-06-0141-01A	0 years, male, white, stage:'--, dead, 313 days	37.7
TCGA-06-0645-01A	0 years, female, white, stage:'--, dead, 175 days	36.8
TCGA-14-0817-01A	0 years, female, white, stage:'--, dead, 164 days	36.8
TCGA-06-0190-01A	0 years, male, white, stage:'--, dead, 317 days	36.7
TCGA-28-5220-01A	0 years, male, white, stage:'--, dead, 388 days	36.3
TCGA-06-0210-01A	0 years, female, white, stage:'--, dead, 225 days	35.4
TCGA-06-0132-01A	0 years, male, white, stage:'--, dead, 771 days	35.2
TCGA-06-2563-01A	0 years, female, white, stage:'--, alive, 932 days	35.0
TCGA-19-2629-01A	0 years, male, white, stage:'--, dead, 737 days	33.5
TCGA-27-1830-01A	0 years, male, white, stage:'--, dead, 154 days	31.3
TCGA-06-2558-01A	0 years, female, white, stage:'--, dead, 380 days	31.2
TCGA-14-0787-01A	0 years, male, asian, stage:'--, dead, 68 days	31.0
TCGA-06-2570-01A	0 years, female, white, stage:'--, alive, 958 days	30.7
TCGA-02-0055-01A	0 years, female, white, stage:'--, dead, 76 days	30.1
TCGA-14-1823-01A	0 years, female, white, stage:'--, dead, 543 days	29.2
TCGA-06-0138-01A	0 years, male, white, stage:'--, dead, 737 days	29.0
TCGA-26-5139-01A	0 years, female, white, stage:'--, alive, 48 days	28.7
TCGA-76-4929-01A	0 years, female, white, stage:'--, dead, 111 days	28.3
TCGA-06-2562-01A	0 years, male, white, stage:'--, dead, 382 days	28.0
TCGA-27-1837-01A	0 years, male, white, stage:'--, dead, 427 days	28.0
TCGA-06-5418-01A	0 years, female, white, stage:'--, dead, 83 days	27.7
TCGA-06-1804-01A	0 years, female, white, stage:'--, dead, 414 days	27.5
TCGA-32-1982-01A	0 years, female, white, stage:'--, dead, 142 days	26.8
TCGA-06-0219-01A	0 years, male, white, stage:'--, dead, 22 days	26.4
TCGA-32-2616-01A	0 years, female, white, stage:'--, dead, 224 days	26.3
TCGA-02-2485-01A	0 years, male, black or african american, stage:'--, alive, 470 days	25.8
TCGA-19-4065-01A	0 years, male, white, stage:'--, alive, 214 days	25.4
TCGA-76-4927-01A	0 years, male, white, stage:'--, dead, 535 days	25.3
TCGA-14-0789-01A	0 years, male, white, stage:'--, dead, 342 days	24.5
TCGA-12-3653-01A	0 years, female, white, stage:'--, dead, 442 days	23.7
TCGA-26-5135-01A	0 years, female, white, stage:'--, dead, 270 days	23.3
TCGA-06-5411-01A	0 years, male, white, stage:'--, dead, 254 days	23.2
TCGA-28-1753-01A	0 years, male, white, stage:'--, alive, 37 days	22.6
TCGA-06-2559-01A	0 years, male, white, stage:'--, dead, 150 days	22.6
TCGA-06-0168-01A	0 years, female, white, stage:'--, dead, 598 days	22.4
TCGA-06-0644-01A	0 years, male, black or african american, stage:'--, dead, 384 days	22.3
TCGA-28-5208-01A	0 years, male, white, stage:'--, dead, 544 days	21.5
TCGA-41-4097-01A	0 years, female, white, stage:'--, dead, 6 days	21.3
TCGA-14-2554-01A	0 years, female, white, stage:'--, dead, 532 days	21.3
TCGA-32-2638-01A	0 years, male, white, stage:'--, dead, 766 days	21.3
TCGA-06-0157-01A	0 years, female, white, stage:'--, dead, 97 days	21.2
TCGA-32-2615-01A	0 years, male, white, stage:'--, dead, 485 days	19.5
TCGA-19-2620-01A	0 years, male, white, stage:'--, dead, 148 days	19.0
TCGA-19-1390-01A	0 years, female, white, stage:'--, dead, 772 days	18.8
TCGA-06-5859-01A	0 years, male, white, stage:'--, alive, 139 days	18.3
TCGA-32-1970-01A	0 years, male, white, stage:'--, dead, 468 days	18.3
TCGA-28-2514-01A	0 years, male, asian, stage:'--, alive, 160 days	18.2
TCGA-12-0616-01A	0 years, female, white, stage:'--, dead, 448 days	17.8
TCGA-28-5204-01A	0 years, male, white, stage:'--, dead, 454 days	17.6
TCGA-06-0747-01A	0 years, male, white, stage:'--, dead, 82 days	17.1
TCGA-12-0619-01A	0 years, male, white, stage:'--, dead, 1062 days	17.0
TCGA-76-4931-01A	0 years, female, white, stage:'--, dead, 279 days	16.4
TCGA-06-0178-01A	0 years, male, white, stage:'--, dead, 2681 days	16.3
TCGA-12-3650-01A	0 years, male, white, stage:'--, dead, 333 days	16.2
TCGA-06-5856-01A	0 years, male, white, stage:'--, dead, 114 days	15.7
TCGA-06-2565-01A	0 years, male, asian, stage:'--, dead, 506 days	15.2
TCGA-06-5413-01A	0 years, male, white, stage:'--, alive, 268 days	15.2
TCGA-02-0047-01A	0 years, male, white, stage:'--, dead, 448 days	15.0
TCGA-06-0125-01A	0 years, female, white, stage:'--, dead, 1448 days	15.0
TCGA-27-2524-01A	0 years, male, white, stage:'--, dead, 231 days	14.5
TCGA-28-2513-01A	0 years, female, white, stage:'--, alive, 222 days	14.2
TCGA-27-1831-01A	0 years, male, white, stage:'--, dead, 505 days	14.2
TCGA-14-1825-01A	0 years, male, white, stage:'--, dead, 232 days	14.1
TCGA-06-0686-01A	0 years, male, white, stage:'--, dead, 432 days	14.0
TCGA-06-5408-01A	0 years, female, white, stage:'--, dead, 357 days	13.8
TCGA-06-0187-01A	0 years, male, white, stage:'--, dead, 828 days	13.7
TCGA-19-2624-01A	0 years, male, white, stage:'--, dead, 5 days	13.6
TCGA-12-0618-01A	0 years, male, white, stage:'--, dead, 395 days	13.3
TCGA-26-1442-01A	0 years, male, white, stage:'--, alive, 953 days	12.8
TCGA-76-4932-01A	0 years, female, white, stage:'--, dead, 1458 days	12.7
TCGA-12-5295-01A	0 years, female, white, stage:'--, dead, 454 days	12.6
TCGA-27-2526-01A	0 years, female, white, stage:'--, dead, 87 days	12.6
TCGA-12-5299-01A	0 years, female, white, stage:'--, dead, 98 days	12.5
TCGA-28-5216-01A	0 years, male, white, stage:'--, alive, 415 days	12.4
TCGA-76-4925-01A	0 years, male, white, stage:'--, dead, 146 days	12.2
TCGA-19-2619-01A	0 years, female, black or african american, stage:'--, alive, 294 days	12.2
TCGA-27-2519-01A	0 years, male, white, stage:'--, dead, 550 days	12.2
TCGA-19-5960-01A	0 years, male, white, stage:'--, dead, 455 days	12.1
TCGA-06-0174-01A	0 years, male, white, stage:'--, dead, 98 days	12.1
TCGA-27-2523-01A	0 years, male, white, stage:'--, dead, 489 days	12.0
TCGA-06-0743-01A	0 years, male, white, stage:'--, dead, 803 days	11.8
TCGA-26-5132-01A	0 years, male, white, stage:'--, alive, 286 days	11.6
TCGA-41-2571-01A	0 years, male, white, stage:'--, dead, 26 days	11.5
TCGA-32-2634-01A	0 years, male, white, stage:'--, alive, 693 days	11.5
TCGA-06-0745-01A	0 years, male, white, stage:'--, dead, 239 days	11.4
TCGA-28-2509-01A	0 years, female, white, stage:'--, alive, 145 days	10.6
TCGA-14-1829-01A	0 years, male, black or african american, stage:'--, alive, 218 days	10.2
TCGA-12-0821-01A	0 years, male, white, stage:'--, dead, 323 days	10.0
TCGA-27-1832-01A	0 years, female, white, stage:'--, dead, 300 days	9.9
TCGA-41-2572-01A	0 years, male, white, stage:'--, dead, 406 days	9.9
TCGA-41-3915-01A	0 years, male, white, stage:'--, dead, 360 days	9.6
TCGA-41-5651-01A	0 years, female, black or african american, stage:'--, dead, 460 days	9.3
TCGA-06-0211-01A	0 years, male, white, stage:'--, dead, 360 days	8.9
TCGA-02-2483-01A	0 years, male, asian, stage:'--, alive, 466 days	8.8
TCGA-28-5209-01A	0 years, female, white, stage:'--, alive, 442 days	8.2
TCGA-26-5134-01A	0 years, male, white, stage:'--, alive, 167 days	7.3
TCGA-06-0156-01A	0 years, male, white, stage:'--, dead, 178 days	7.3
TCGA-08-0386-01A	0 years, male, white, stage:'--, dead, 548 days	7.1
TCGA-06-0750-01A	0 years, male, white, stage:'--, dead, 28 days	7.0
TCGA-27-1835-01A	0 years, female, white, stage:'--, dead, 648 days	6.6
TCGA-27-2528-01A	0 years, male, white, stage:'--, dead, 480 days	6.0
TCGA-06-0744-01A	0 years, male, white, stage:'--, dead, 1426 days	5.7
TCGA-06-5417-01A	0 years, female, white, stage:'--, alive, 155 days	4.7
TCGA-19-2625-01A	0 years, female, white, stage:'--, dead, 124 days	4.7
TCGA-27-2521-01A	0 years, male, white, stage:'--, dead, 510 days	4.5
TCGA-28-5218-01A	0 years, male, white, stage:'--, dead, 157 days	4.4
TCGA-15-0742-01A	0 years, male, white, stage:'--, dead, 419 days	4.2
TCGA-26-5133-01A	0 years, male, white, stage:'--, alive, 452 days	3.6
TCGA-32-4213-01A	0 years, female, white, stage:'--, alive, 604 days	3.4
TCGA-14-0871-01A	0 years, female, white, stage:'--, dead, 880 days	3.3
TCGA-06-5416-01A	0 years, female, white, stage:'--, alive, 204 days	2.1
TCGA-06-2569-01A	0 years, female, black or african american, stage:'--, alive, 13 days	1.3
TCGA-12-3652-01A	0 years, male, white, stage:'--, dead, 1062 days	1.0
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GLIOBLASTOMA MULTIFORME (VALIDATION) - Interactive survival scatter plotⁱ

& Survival analysisⁱ

OLR1 is not prognostic in Glioblastoma Multiforme (validation)

Stage:

Survival analysis

Current cut offⁱ :

Best expression cut offⁱ : 17.82

Median expressionⁱ : N/A

Median follow up timeⁱ : N/A

P scoreⁱ N/A

3-year survival highⁱ N/A

3-year survival lowⁱ N/A

TCGA RNA samplesⁱ

Average TPM

15.5

Number of samples

Samples

Sample	Description	TPM
AK030	N, NA, stage:NA, dead, 120 days	114.0
AK167	N, NA, stage:NA, dead, 180 days	47.8
AK081	N, NA, stage:NA, dead, 180 days	33.6
AK003	N, NA, stage:NA, dead, 600 days	33.4
AK072	N, NA, stage:NA, dead, 150 days	29.0
AK195	N, NA, stage:NA, dead, 390 days	27.2
AK188	N, NA, stage:NA, alive, 420 days	26.5
AK124	N, NA, stage:NA, alive, 1920 days	24.9
AK134	N, NA, stage:NA, dead, 360 days	23.9
AK236	N, NA, stage:NA, dead, 360 days	23.7
AK183	N, NA, stage:NA, alive, 360 days	22.9
AK099	N, NA, stage:NA, dead, 420 days	21.6
AK227	N, NA, stage:NA, alive, 300 days	21.5
AK066	N, NA, stage:NA, alive, 960 days	20.7
AK103	N, NA, stage:NA, alive, 1320 days	20.5
AK085	N, NA, stage:NA, alive, 660 days	20.4
AK015	N, NA, stage:NA, alive, 720 days	20.4
AK088	N, NA, stage:NA, dead, 360 days	20.3
AK185	N, NA, stage:NA, alive, 330 days	19.6
AK006	N, NA, stage:NA, dead, 360 days	18.9
AK076	N, NA, stage:NA, alive, 660 days	18.1
AK071	N, NA, stage:NA, dead, 540 days	17.6
AK005	N, NA, stage:NA, dead, 240 days	17.2
AK153	N, NA, stage:NA, dead, 240 days	17.1
AK158	N, NA, stage:NA, dead, 360 days	16.9
AK055	N, NA, stage:NA, dead, 240 days	16.6
AK100	N, NA, stage:NA, alive, 960 days	13.2
AK133	N, NA, stage:NA, dead, 360 days	12.8
AK079	N, NA, stage:NA, dead, 210 days	12.5
AK091	N, NA, stage:NA, dead, 210 days	11.9
AK178	N, NA, stage:NA, dead, 240 days	10.2
AK173	N, NA, stage:NA, dead, 180 days	10.1
AK216	N, NA, stage:NA, dead, 360 days	8.5
AK098	N, NA, stage:NA, dead, 660 days	8.4
AK199	N, NA, stage:NA, alive, 1860 days	8.2
AK068	N, NA, stage:NA, alive, 360 days	7.9
AK139	N, NA, stage:NA, dead, 270 days	7.8
AK149	N, NA, stage:NA, dead, 420 days	7.7
AK123	N, NA, stage:NA, dead, 180 days	7.6
AK089	N, NA, stage:NA, dead, 330 days	7.0
AK231	N, NA, stage:NA, dead, 240 days	6.8
AK035	N, NA, stage:NA, dead, 210 days	6.4
AK165	N, NA, stage:NA, alive, 360 days	6.2
AK053	N, NA, stage:NA, dead, 300 days	5.5
AK226	N, NA, stage:NA, dead, 360 days	5.2
AK041	N, NA, stage:NA, alive, 810 days	4.9
AK218	N, NA, stage:NA, dead, 180 days	4.9
AK074	N, NA, stage:NA, alive, 300 days	4.9
AK156	N, NA, stage:NA, dead, 330 days	4.7
AK102	N, NA, stage:NA, alive, 1740 days	3.6
AK142	N, NA, stage:NA, alive, 90 days	3.4
AK213	N, NA, stage:NA, alive, 2010 days	3.1
AK117	N, NA, stage:NA, dead, 210 days	3.0
AK051	N, NA, stage:NA, dead, 360 days	2.2
AK205	N, NA, stage:NA, dead, 120 days	2.2
AK049	N, NA, stage:NA, dead, 180 days	2.1
AK043	N, NA, stage:NA, alive, 660 days	1.6
AK002	N, NA, stage:NA, dead, 570 days	1.3
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GLIOBLASTOMA MULTIFORME - Protein relative expression (CPTAC)

Number of samples

110

Samples

Sample ID	Sample type	nRPX
CPT0206330003	Tumor	N/A
CPT0186100003	Tumor	N/A
CPT0123530003	Tumor	N/A
CPT0201710003	Tumor	N/A
CPT0216920008	Tumor	N/A
CPT0218890004	Tumor	N/A
CPT0224390004	Tumor	N/A
CPT0204390003	Normal	N/A
CPT0093590003	Tumor	N/A
CPT0167750004	Tumor	N/A
CPT0204400003	Normal	N/A
CPT0079790003	Tumor	N/A
CPT0218690004	Tumor	N/A
CPT0204370003	Normal	N/A
CPT0161730003	Tumor	N/A
CPT0168720003	Tumor	N/A
CPT0206880003	Tumor	N/A
CPT0189650004	Tumor	N/A
CPT0204350003	Normal	N/A
CPT0168380003	Tumor	N/A
CPT0205890003	Tumor	N/A
CPT0217880003	Tumor	N/A
CPT0206560003	Tumor	N/A
CPT0087680003	Tumor	N/A
CPT0206000004	Tumor	N/A
CPT0218670003	Tumor	N/A
CPT0204340003	Normal	N/A
CPT0189850004	Tumor	N/A
CPT0224330003	Tumor	N/A
CPT0218330004	Tumor	N/A
CPT0167640003	Tumor	N/A
CPT0002410011	Tumor	N/A
CPT0093450003	Tumor	N/A
CPT0206110003	Tumor	N/A
CPT0217430008	Tumor	N/A
CPT0217000004	Tumor	N/A
CPT0196850003	Tumor	N/A
CPT0189250003	Tumor	N/A
CPT0189570004	Tumor	N/A
CPT0162020003	Tumor	N/A
CPT0167530003	Tumor	N/A
CPT0207030003	Tumor	N/A
CPT0127420003	Tumor	N/A
CPT0205670004	Tumor	N/A
CPT0208980003	Tumor	N/A
CPT0206780003	Tumor	N/A
CPT0182500003	Tumor	N/A
CPT0217710008	Tumor	N/A
CPT0168830003	Tumor	N/A
CPT0182550003	Tumor	N/A
CPT0189460003	Tumor	N/A
CPT0167860004	Tumor	N/A
CPT0125220003	Tumor	N/A
CPT0171580008	Tumor	N/A
CPT0087730003	Tumor	N/A
CPT0104330003	Tumor	N/A
CPT0168590003	Tumor	N/A
CPT0205450004	Tumor	N/A
CPT0219080004	Tumor	N/A
CPT0087570003	Tumor	N/A
CPT0162140003	Tumor	N/A
CPT0175060003	Tumor	N/A
CPT0205780003	Tumor	N/A
CPT0218960004	Tumor	N/A
CPT0064650003	Tumor	N/A
CPT0125570003	Tumor	N/A
CPT0190360004	Tumor	N/A
CPT0206670004	Tumor	N/A
CPT0204380003	Normal	N/A
CPT0092440003	Tumor	N/A
CPT0162100003	Tumor	N/A
CPT0221180003	Tumor	N/A
CPT0225760003	Tumor	N/A
CPT0093510003	Tumor	N/A
CPT0190240004	Tumor	N/A
CPT0228220003	Tumor	N/A
CPT0204410003	Normal	N/A
CPT0224540004	Tumor	N/A
CPT0209440003	Tumor	N/A
CPT0089150003	Tumor	N/A
CPT0204360003	Normal	N/A
CPT0168080003	Tumor	N/A
CPT0078580003	Tumor	N/A
CPT0127480003	Tumor	N/A
CPT0204420003	Normal	N/A
CPT0218830004	Tumor	N/A
CPT0225730003	Tumor	N/A
CPT0093360003	Tumor	N/A
CPT0168270003	Tumor	N/A
CPT0206450003	Tumor	N/A
CPT0207090003	Tumor	N/A
CPT0104220003	Tumor	N/A
CPT0224600003	Tumor	N/A
CPT0087950003	Tumor	N/A
CPT0204330003	Normal	N/A
CPT0199770003	Tumor	N/A
CPT0205570003	Tumor	N/A
CPT0093550003	Tumor	N/A
CPT0167970003	Tumor	N/A
CPT0182580003	Tumor	N/A
CPT0168480003	Tumor	N/A
CPT0206230003	Tumor	N/A
CPT0189750004	Tumor	N/A
CPT0217100003	Tumor	N/A
CPT0064890003	Tumor	N/A
CPT0217190003	Tumor	N/A
CPT0218770003	Tumor	N/A
CPT0125510003	Tumor	N/A
CPT0217060003	Tumor	N/A
CPT0071100003	Tumor	N/A
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GLIOMA - Protein expressionⁱ

Pending cancer tissue analysis