Multiplex tissue profiling - protein localization in single-cell types

The Tissue resource has implemented a novel protocol for studying protein localization in single-cell types, cell states and subcellular structures in human tissue samples, utilizing multiplex immunohistochemistry-based fluorescence imaging. With the help of single-cell transcriptomics and an iterative staining-stripping method, seven antibody panels have been developed to profile eight different tissues. In version 24.0, 1021 proteins have been analyzed using multiplex tissue profiling. More information about the generation of the multiplex tissue profiling data can be found on the Methods page.

Learn about:

  • Spatial analysis at a resolution not obtained by regular immunohistochemistry
  • Overlap in expression between uncharacterized proteins and specific cell type markers
  • Protein localization in single-cell types, cell states and subcellular structures

MULTIPLEX TISSUE PROFILINGi
Explore the proteomes of cell types and cell states

The different organs and their antibody panels used for multiplex tissue profiling can be explored in this interactive database, including catalogs of proteins expressed in specific cell types, states, and structures.

image/svg+xml human body features 2009-05-27 Sjef text : Kevin (tsaitgaist) REDON http://commons.wikimedia.org/wiki/File:Human_body_features.svg leukocyte hippocampus nasal septum oral cavity prostate gland
image/svg+xml leukocyte artery B cell T cell natural killer cell monocyte platelet hippocampus oral cavity tongue retina


Multiplex antibody panels

An interactive lollipop plot showing the number of proteins profiled with each antibody panel. Click on the hexagons to obtain the corresponding list of proteins.

12116223527689102178177177178178Salivary gland Kidney Testis - SpermatogoniaTestis - SpermatocytesTestis - SpermatidsTestis - Sertoli cells Bronchus - Ciliated cellsNasopharynx - Ciliated cellsCervix - Ciliated cellsEndometrium - Ciliated cellsFallopian tube - Ciliated cells050100150200250300Proteins


Spatial localization based on multiplex tissue profiling

List presenting the number of proteins mainly located to different cell types/structures based on manual annotation of images stained with multiplex tissue profiling.

Antibody panel
Tissue
Main location
# proteins
Salivary gland Salivary gland Ionocytes 23
Salivary gland Large ducts 46
Salivary gland Mucus acini 47
Salivary gland Serous acini 88
Salivary gland Small ducts 54
Kidney Kidney Collecting ducts 53
Kidney Distal tubules 54
Kidney Endothelia 26
Kidney Podocytes 29
Kidney Proximal tubules 60
Spermatogonia Testis Spermatocytes 1 50
Testis Spermatogonia 0 134
Testis Spermatogonia 1 68
Testis Spermatogonia 2-3 81
Testis Spermatogonia 4 88
Spermatocytes Testis Spermatids early 80
Testis Spermatids late 28
Testis Spermatocytes 1 98
Testis Spermatocytes 2 102
Testis Spermatocytes 3 201
Spermatids Testis Spermatids early 1 36
Testis Spermatids early 1 & Spermatids early 2 28
Testis Spermatids early 2 16
Testis Spermatids late 1 29
Testis Spermatids late 2 63
Testis Spermatids late 2 & Spermatids late 3 45
Testis Spermatids late 3 51
Sertoli cells Testis Sertoli cytoplasm 53
Testis Sertoli membrane 13
Testis Sertoli nuclei 44
Testis Spermatids 7
Testis Spermatogonia and spermatocytes 26
Ciliated cells Bronchus Cilia axoneme 122
Bronchus Cilia basal body 4
Bronchus Cilia transition zone 22
Bronchus Cytoplasm 58
Bronchus Nucleus 25
Nasopharynx Cilia axoneme 101
Nasopharynx Cilia basal body 4
Nasopharynx Cilia transition zone 21
Nasopharynx Cytoplasm 49
Nasopharynx Nucleus 27
Cervix Cilia axoneme 90
Cervix Cilia basal body 1
Cervix Cilia transition zone 20
Cervix Cytoplasm 40
Cervix Nucleus 22
Endometrium Cilia axoneme 82
Endometrium Cilia basal body 2
Endometrium Cilia transition zone 16
Endometrium Cytoplasm 28
Endometrium Nucleus 17
Fallopian tube Cilia axoneme 108
Fallopian tube Cilia basal body 2
Fallopian tube Cilia transition zone 23
Fallopian tube Cytoplasm 42
Fallopian tube Nucleus 20