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Module 8: Immunohistochemistry Microscopy

Group leader: Dr Kenneth Wester

Personnel: Dijana Djurenovic (technician), Linda Oskarsson (technician) and Marcus Runeson (technician).

Responsibility: (i) Assessing optimal immunostaining protocol and validation of antibody target specificity (ii), maintaining immunostaining reproducibility and quality, (iii) coordinating evaluation of antibodies submitted by commercial vendors and academic scientists.

Description: Optimal antibody dilution and target specificity is assessed by microscopical examination. Available information from both gene and protein public databases, as well as in-house technical validation, such as protein arrays and Western blots, are considered in the decission process. For each approved antibody, a final immunostaining protocol is defined and subsequently applied to the full-scale TMAs.

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