Human Protein Atlas

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Time frames and rules for the collaborative projects

If approved, confirmation of antibodies to be included in the HPA pipeline will be sent to the applicant (collaborator). This information will be kept confidential by the HPA. The collaborator is asked to send a vial of the approved antibodies to the HPA. A minimum amount of 50 µg or equivalent will be needed for each antibody aliquot, delivered in appropriate tubes (preferably 1-2 ml volume with screw cap) and properly labeled.
Each antibody is tested according to HPA-standard procedures, i.e., on Western blots and on a special designed TMA initially using two antibody-dilutions and heat epitope retrieval in citrate buffer.
The results of the antibody validation and the decision to approve or not approve the antibody for the Protein Atlas will be sent to the antibody provider. This information will also be kept confidential by the HPA.
Each antibody approved by HPA will subsequently be immunostained on eight TMAs representing 576 tissues and manually annotated.
Confirmation of successful tissue profiling (including annotation) will be sent to the collaborator together with instruction (password to the internal web database) how to access the annotated tissue array images with the results from the antibody. The collaborator is free to use this information for his/her own research or marketing (in the case of commercial vendors).

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