Human Protein Atlas

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protein atlas

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submission of antibodies

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Quality assurance of antibodies

A documentation of the validation should be submitted preferably through a web-link (product data sheet and/or reference) or alternatively as a pdf-file (with results, reference and/or product data sheet). Antibodies should be validated by at least one of the following two methods:

Western blot (with relevant human tissue extracts and /or cells)
Immunohistochemistry (IHC with relevant human tissue and/or cells)

Examples of other suitable validation assays are:

Antigen-based assays (ELISA, protein arrays or similar protein assay)
Adsorption test (in combination with IHC)
Flow sorting
Immunocapture results ("pull-down" followed by suitable analysis)

Flow of information
The antibody provider (academic or commercial) will obtain the results of the quality assurance. Confirmation of successful tissue profiling (including annotation) will be sent to the collaborator together with an instruction (password to the internal web database) on how to access the results from the protein profiling. Information about non-approved antibodies will only be communicated to the antibody provider (and will not be published). For academic collaborators, the data will be stored at least six months on the internal web atlas before transfer to the publicly available Protein Atlas (www.proteinatlas.org). Each approved antibody on the public database will be directly linked from the Protein Atlas to the collaborators web-home page.

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