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- [New] A FAQ document (Frequently Asked Questions) has been added and is available as a link on the bottom of each page.
- [New] Each image has now been complemented with more tissue information such as T-number and M-number as defined by SNOMED.
- [Change] The term Staining intensity has been changed to Protein expression on several pages.
- [Change] The ETS1-associated protein 1 (EAP1) antibody HPA008797 has been removed due to weak and unspecific staining. An alternative antibody HPA008736 is available on the Protein Atlas.
- [Change] The Myeloid zinc finger 1 antibody (HPA001757) stained images have been omitted and replaced with images from a more recent staining with the same antibodies.
- [Change] The E3 ubiquitin-protein ligase Praja1 antibody (HPA000595) stained images have been omitted and replaced with images from a more recent staining with the same antibodies.
- [Change] The WD repeat protein 13 antibody (HPA000239) has been omitted due to non-reliable protein expression patterns (IHC), showing mainly cytoplasmic positivity. An alternative antibody (HPA000913), displaying distinct nuclear staining, has been added to the Protein Atlas.
- [Change] Medullar cells has been removed for adrenal gland.
- [Change] Lymphoid tissue has been renamed to lymphoid cells in appendix.
- [Change] Bone marrow poetic cells has been renamed to bone marrow poietic cells.
- [Change] Surface epithelial cells has been renamed to respiratory epithelial cells in bronchus.
- [Change] Non-neuronal cells has been renamed to glial cells in cerebral cortex.
- [Change] Surface epithelial cells (squamous) has been renamed to squamous epithelial cells in cervix, uterine.
- [Change] Endometrium has been renamed to corpus, uterine.
- [Change] Cells in endometrial stroma/ECM has been renamed to cells in endometrial stroma in corpus, uterine.
- [Change] Cells in myometrium/ECM has been removed for corpus, uterine.
- [Change] Surface epithelial cells has been renamed to squamous epithelial cells in esophagus.
- [Change] Non-neuronal cells has been renamed to glial cells in hippo campus.
- [Change] Cells in tubuli has been renamed to cells in tubules in kidney.
- [Change] Non-neuronal cells has been renamed to glial cells in lateral ventricle.
- [Change] Follicle cells (cortex) has been renamed to reaction center cells in lymph node.
- [Change] Non-follicle cells (paracortex) has been renamed to lymphoid cells outside reaction centra in lymph node.
- [Change] Surface epithelial cells has been renamed to respiratory epithelial cells in nasopharynx.
- [Change] Surface epithelial cells has been renamed to squamous epithelial cells in oral mucosa.
- [Change] Exocrine pancreas has been renamed to exocrine glandular cells in pancreas.
- [Change] Cells in ductus seminiferus has been renamed to cells in seminiferus ducts in testis.
- [Change] Follicle cells (cortex) has been renamed to reaction center cells in tonsil.
- [Change] Non-follicle cells (paracortex) has been renamed to lymphoid cells outside reaction centra in tonsil.
- [Change] Surface epithelial cells has been renamed to squamous epithelial cells in tonsil.
- [Change] Surface epithelial cells has been renamed to urothelial cells in urnary bladder.
- [Change] Surface epithelial cells has been renamed to squamous epithelial cells in vagina.
- [Change] Surface epithelial cells has been renamed to squamous epithelial cells in vulva/anal skin.
- [Change] Colo-rectal cancer has been renamed to colorectal cancer.
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- [New] Third major release of the Human Protein Atlas with more than 3.000 antibodies and more than 2.800.000 images.
- [New] Section for immunoflourescence staining of cell lines and confocal scanned images.
- [New] Advanced search functionality enabling expression pattern search.
- [New] Sort IHC tissues by alphabetic or histogenic order.
- [New] Antibody specifility diagrams are now available for each HPA antibody based on array experiments. The diagrams can be found on the antibody info page.
- [New] Western blot images are showed on selected antibodies on the antibody info page.
- [Change] The SMC1 alpha protein antibody (HPA002855) has been omitted due to weak immunostaining. An alternative antibody (HPA005499), showing a more distinct immunostaining pattern, has been added to the Protein Atlas.
- [Change] The TNF-R2 antibody (CAB002614) has been omitted due to a nuclear staining pattern. An alternative antibody (HPA004796), showing cytoplasmic and membranous positivity, has been added to the Protein Atlas.
- [Change] The IL-1R-1 antibody (HPA001237) has been omitted due to a nuclear staining pattern. An alternative antibody (CAB007779) has been added to the Protein Atlas.
- [Change] The LTF antibody (HPA001833) has been omitted due to deficient immunostaing with expected extra-cellular positivity. An alternative antibody (CAB008645) has been added to the Protein Atlas.
- [Change] The FERM and PDZ domain-containing protein 3 antibody (HPA000830) has been omitted due to unspecific staining pattern.
- [Change] The MMP-3 antibody (CAB002747) has been omitted due to low quality immunostaining pattern with only sparse positivity in mucous producing cells.
- [Change] The AMOT antibody (CAB003708) has been omitted due to lack of expected positivity in endothelial cells.
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- [New] Second major release of the Human Protein Atlas with more than 1.500 antibodies and 1.000.000 images.
- [New] Cell profiles have been added to complement the tissue profiles. The new profiles consist of 47 cell lines and 12 primary cells that have been stained with HPA antibodies and evaluated using automated image analysis. The results are presented at the bottom of the expression profile-page for almost every antibody.
- [Change] Due to late finding of an incorrect molecular weight of the antigens for HPA001340 and HPA001660 these antibodies were removed.
- [Change] Due to weak and unspecific staining using the CDC2 antibody, CAB002435, a new immunostaining and subsequent annotation has been performed using the alternative antibody, CAB003799.
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- [Change] The underlying algorithms for color code representation of
negative, weak, moderate and strong staining has been modified. In the updated version,
an immunostaining of less than 25% of the recorded cell type has been given less weight
compared to the previous version. The result provides a more accurate display of the
represented immunostained cell population.
- [Change] In the previous version, tissues with one or several cell types missing were shown as a negative (white) color code.
In the present version, this has been changed so that all cell types now displayed as negative (white) correctly represent existing cell types that lack immunohistochemical staining.
The color code black (Not representative) has now been extended to include both not representative images as well as images where cell types are missing.
The overall result will be an increase of displayed cell types as "Not representative" (black).
- [Change] The explanation for a black color code on the summary page has been
changed from "No images" to "Not representative"
- [Change] A new design of the table displaying the splice variants for each gene.
- [Update] Due to a weak and unspecific staining of the Integrin
antibody, CAB003434, a new immunostaining has been performed using the same
antibody. Images and annotation has been updated and the old version,
CAB000127, has been removed.
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- [Change] Changes have been made in the presentation of annotated localization patterns. Previous version included 4 different parameters for describing subcellular localization of protein expression: i) nuclear, ii) cytoplasmic, iii) membrane and iv) extra cellular. In the present version cytoplasmic and membraneous localization have been merged so that the output parameters now are i) nuclear, ii) cytoplasmic and/or membraneous. Due to the level of resolution using enzyme-based immunohistochemistry it is not possible to distinguish between cytoplasmic and membraneous localization in cells with a small cytoplasm. Cytoplasmic and membraneous localization has consequently been changed to one shared output parameter. Extra cellular localization has been omitted as a localization parameter given for a cell type.
Corresponding text concerning annotation in “about protein atlas” has been modified accordingly.
- [Change] A new summary page has been created. Previous version included two different summary pages, one for normal tissues and one for cancer tissues as well as a summary page containing all antibody information. In the present version the summary page contains a short gene description and a summary of both normal and cancer tissues.
- [New] A new tab on the start page has been inserted. The new tab represents a “submission of antibodies” and contains a call for antibodies, information and an application form. Previous version included a tab “collaborations” which has been omitted.
- [Update] Reliabilty scores have been curated for a subset of antibodies in the protein atlas since new data has allowed for detailed validation. A validation comment text has been added in these cases.
- [New] The possibility to reference or link into a particular antibody in the Protein Atlas has now been simplified. The Protein Atlas now supports links in the following two new formats: HUGO ID and Ensembl gene ID. Examples of the new URLs:
http://www.proteinatlas.org/tissue_profile.php?hugo_id=bmx
or
http://www.proteinatlas.org/tissue_profile.php?ensembl_gene_id=ENSG00000102010
- [New] Version history (this text) has been added as text under the tab “about protein atlas”.
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- First release of the Protein Atlas. Atlas content: 718 antibodies and 413,568 images
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home | about protein atlas - release history
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