Subcellular - NES - The Human Protein Atlas

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NES

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Field

Cluster

Pathway

SUMMARY

TISSUE

BRAIN

SINGLE CELL

SUBCELL

CANCER

BLOOD

CELL LINE

STRUCT & INT

NES

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GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NES
Gene descriptionⁱ Full gene name according to HGNC.	Nestin
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s).	Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Main locationⁱ Main subcellular location(s) and reliability score(s) for the encoded protein(s) in human cells. The main location(s) may be characterized by presence in all tested cell lines and/or ihigher staining intensity compared to the potential additional location(s). If available, links to overrepresentation analyses in Reactome, a free, open-source, curated and peer reviewed biological pathway database, are provided. An analysis is done for the corresponding gene set of the proteome localizing to the main and additional locations of the protein on this page, respectively.	Localized to the Intermediate filaments (enhanced)
Single-cell variationⁱ Single-cell variations in protein expression levels or distribution observed between cells within the same images of human cells stained by immunocytochemistry.	Single-cell variation in protein expression observed.
Cell cycle dependencyⁱ Cell-cycle dependance of single-cell varations in RNA and/or protein expression as observed in an additional assay for characterizing single-cell varations. "NA" indicates a lack of such data.	Variation in protein and transcript expression do not correlate to the cell-cycle.
GFPⁱ Validation score for the subcellular location(s) of the encoded proteins(s) according to a GFP assay.	The location(s) are Enhanced based on analysis of GFP-tagged protein.
Reliability scoreⁱ Overall gene reliability score for the subcellular location(s) of the encoded protein(s). The score indicates the level of reliability of the annotated protein localization based on agreement with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database and presence of antibody validation assays for immunocytochemistry.	Enhanced
Antibodiesⁱ Antibodies used for this assay. Click on an antibody for more information.	CAB005889 , HPA006286 , HPA026111
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ASSAYSⁱ

Summaryⁱ

Localized to the intermediate filaments.

Main locationⁱ

Intermediate filaments (enhanced)

Human cell lines

Single cell variation

GFP

RNA expression

HUMAN CELL LINESⁱ


	Toggle channelsⁱ Buttons for turning on and off the different channels in the multi-color images. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel. The object toggle shows the computational segmentation of the cells used for further analysis in the HPA project. For samples where cell cycle dependency for the protein is suggested according to a correlation assay the predicted cell cycle position of each cell is displayed when using the object toggle.	Low High

Antibodyⁱ Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.	Cell lineⁱ Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.	Cell line RNA Expression (nTPM)ⁱ RNA expression level in the cell line based on normalised RNA sequencing data. Genes with a value above 1 NX are considered to be expressed.	Locationⁱ Observed subcellular location(s).	Single-cell variationⁱ Observed single-cell variation(s) in the staining pattern. Variation in protein expression levels can be observed as varaiations in the intensity of the immunofluorescent signal, while spatial variations can be observed as variations in subcellular distribution of the protein.
HPA026111	AF22	586.0	Intermediate filaments
HPA026111	U-251MG	136.0	Intermediate filaments	Yes
HPA026111	U2OS	47.0	Intermediate filaments	Yes
CAB005889	U2OS	47.0	Intermediate filaments	Yes
HPA006286	AF22	586.0	Intermediate filaments
HPA006286	Rh30	492.6	Intermediate filaments
HPA006286	U2OS	47.0	Intermediate filaments	Yes

SINGLE CELL VARIATIONⁱ

Summaryⁱ

Variation in protein and transcript expression do not correlate to the cell-cycle.

Locationⁱ

Intermediate filaments

Protein expression across cell cycleⁱ

HPA026111: U-2 OS FUCCI	HPA026111: U-2 OS FUCCI
Toggle channelsⁱ Buttons for turning on and off the different channels in the multi-color images.

RNA expression across cell cycleⁱ

GFPⁱ

Antibodyⁱ

HPA006286

Validationⁱ

N-terminal tag
BAC cell line: 5822
Enhanced: Antibody staining overlaps with GFP tagged protein

Antibodyⁱ

HPA026111

Validationⁱ

N-terminal tag
BAC cell line: 5822
Enhanced: Antibody staining overlaps with GFP tagged protein


	Toggle channelsⁱ Buttons for turning on and off the different channels in the multi-color images. The HPA-antibody staining targeting the protein of interest is shown in green and the staining of the GFP-tagged protein in purple. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel.	Low High

Antibodyⁱ Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.	Cell lineⁱ Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.	BAC idⁱ BAC cell line used in the analysis. BAC cell lines have been obtained from the Hyman lab. The untransfected HeLa control cells are referred to as "normal".	Tagⁱ The GFP construct can be tagged to the target protein at either the N- or C-terminal, for some genes both versions are available.	Location antibody stainingⁱ Observed subcellular location(s) annotated for HPA-antibody targeting the endogenous protein.	Location tagged proteinⁱ Observed subcellular location(s) for the antibody targeting the GFP-tagged version of the protein.
HPA006286	HeLa	5822	N-terminal	Intermediate filaments	Intermediate filaments
HPA006286	HeLa	Normal	-	Intermediate filaments	-
HPA026111	HeLa	5822	N-terminal	Intermediate filaments	Intermediate filaments
HPA026111	HeLa	Normal	-	Intermediate filaments	-

RNA EXPRESSION OVERVIEWⁱ

Organ

Origin